Create Primer List
In this step we will create a primer list of left & right primers using the Bio-Informatics tool Primer3
Here we need to set
a.) the 'Task'-dropdown menu from 'generic' to "
pick_primer_list",
b.) the 'Included Region' to the Hypervariable Region I "
15919, 650" (our target sequence Starting at basepair position 15919 with a length 650)
c.) the 'Number To Return' (Number of primer sequences to return) to "
50".
Cut and paste the
RSRS sequence into the window.
Leave all other parameters as is and click
'Pick Primers'