Trouble Shoot Virtual PCR

Your PCR product will show up in a pop-up window and should look similar to this image

       

If Virtual PCR didn't work

- Check position of left and right primer
        Left primer starting position plus length should be lower than first mutation
        Right primer starting position minus length should be higher than last mutation

- Did you input the Correct Sequence?
        The Virtual-PCR Form should look similar to this image.
The first window should have your haplogroup sequence starting with ">hap " and then your primer pair.