Worksheet Section 1 >>

Consider the figure shown above when answering the following questions.

1. Which direction is the reverse primer read? 5' to 3' or 3' to 5'?

2. Which strand of the DNA (top or bottom) will the forward primer ("F") hybridize to during the PCR reaction?

3. Will the DNA synthesized starting from the forward primer be the same sequence as the top strand or the bottom strand?

4. Will the DNA synthesized starting from the reverse primer be the same sequence as the top strand or the bottom strand?

5. Why would the sequence of the control region be more variable than a protein coding region?
        Please limit your answer to 6 sentences.