Analyzing Primer Pair Statistics
13. Please note the Primer Pair Statistic values in your document (cut & Paste).
Base pair position of first mtDNA mutation of working haplogroup :___________
Base pair position of last mtDNA mutation of working haplogroup :___________
Left primer "start" position plus primer length should be lower than base pair position for the first expected mutation.
Right primer "start" position minus primer length should be higher than the base pair position for the last expected mutation.
- Here is an example of the "Check-Primer" Output-table
PRIMER PICKING RESULTS FOR hap RSRS
No mispriming library specified
Using 1-based sequence positions
WARNING: Left primer is unacceptable: Tm too low; Right primer is unacceptable: High hairpin stability (thermod. approach)
OLIGO start len tm gc% any_th 3'_th hairpin seq
LEFT PRIMER 15972 20 56.91 50.00 0.00 0.00 0.00 TAACTCCACCATTAGCACCC
RIGHT PRIMER 16520 20 59.75 60.00 0.00 0.00 50.91 GGCCCTGAAGTAGGAACCAG
SEQUENCE SIZE: 16569
INCLUDED REGION SIZE: 16569
PRODUCT SIZE: 549, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00