Table E.1. A Simple Set of Rules for Primer Design (Adapted from Innis and Gelfand, 1991)
14. How would you assess the primer quality,
based on your primer-3 output and table E.1 of guidelines from the lab manual (See table above)?
15. What is your PCR product size?
16. How many of your mtDNA mutation sites are included in your PCR product?
17. Can you determine your haplogroup from this PCR product?
(i.e. does the product size include the positions of the expected mutations that define your haplogroup)?
Why or why not?