18. Using BLAST compare your PCR product to the RSRS reference sequence and list the mutations below.
How many of the expected mutations do you find?
19. Do you think you selected a good primer pair? Why or why not?
Review Table E.1 in Lab Manual for some guidelines
20. Are the melting temperatures/length of your two primers similar or different?
How could the melting temperature/length affect your PCR amplification?
21. Do you think this is a good universal primer (able to amplify all haplogroups)?
Why or why not?
