BLAST PCR Product Against RSRS

In this step we will compare our haplogroup specific PCR-product with the RSRS reference sequence using the Bio-Informatics tool BLAST.

Here we need to
a.) Goto "NCBI BLAST"-Website
b.) Select "Nucleotide BLAST"-tile.
c.) Select checkbox "Align two or more Sequences".
d.) Copy and paste PCR Product from Virtual PCR (Step 6) into "Enter Query Sequence"-window.
e.) Copy and paste RSRS-Sequence into "Enter Subject Sequence"-window.
f.) Click "BLAST"-button.

Next Action: Go To "NCBI BLAST"-Website



If this is successful you will get a BLAST-Result window.

Click on the "Graphics"-link in the "Descriptions"-tab view.

Zoom in on the end of the RSRS-Sequence and explore the mutations SNPs of your haplogroup. Go back to Section 6 Answer questions #18-21

What to Expect:TroubleShoot.