Run Virtual PCR

In this step we will "run" our PCR using your haplogroup specific DNA and the haplogroup specific primers you designed using the Bio-Informatics tool SMS.
Here we need to
a.) populate the sequence field with the mtDNA Sequence of your Haplogroup (select your haplogroup on map, then copy and paste),
b.) copy and paste your selected primers into the primer fields.
        You can relabel the names ("T7 & T3") to "left primer" and "right primer".
        "first primer" here is the left primer and "second primer" refers to the right primer.
c.) click "Submit".

If this is succssful you will get a "PCR-Product" with a defined length in a pop up window.
Check that length of the PCR Product with your predicted PCR product size on Section 5 Question # 15.

No PCR Product? TroubleShoot.

Next Action:BLAST PCR Product.

Cut PCR product from pop-up window, open Blast and paste it into the first window.